Journal: Frontiers in Oncology
Article Title: Long Non-Coding RNA CD27-AS1-208 Facilitates Melanoma Progression by Activating STAT3 Pathway
doi: 10.3389/fonc.2021.818178
Figure Lengend Snippet: The expression of lncRNA CD27-AS1-208, which locates in the nucleus, is increased during melanoma progression and significantly correlation with Ki-67 level. (A) Validation of differentially-expressed lncRNAs with a new cohort of samples. (B) The expression level of CD27-AS1-208 in melanocytes and melanoma cell lines. (C, D) The expression level of CD27-AS1-208 in the cytoplasm and nucleus of melanoma cells. (E) The location of CD27-AS1-208 in primary melanoma and nevus. (F) Immunofluorescence staining of Ki-67 in indicated melanoma tissues. (G) Correlation between CD27-AS1-208 level and Ki67 staining in melanoma tissue. * P < 0.05, ** P < 0.01, *** P < 0.001, NHEM or cytoplasm as control; # HMS as control, # P < 0.05, ## P < 0.01; & PIG1 as control, & P < 0.05, && P < 0.01. Scale bar = 10μm. ns., not significant.
Article Snippet: The immortalized normal human epidermal melanocyte cell line PIG1 was a gift from Dr. Caroline Le Poole, Loyola University Chicago, Maywood, IL ( ).WM793B, WM35, A375, WM-266, A2058, 451Lu, Hs294T, 1205Lu, SK-MEL-1 and SK-MEL-5 were purchased from American Type Culture Collection (ATCC) in 2014.
Techniques: Expressing, Biomarker Discovery, Immunofluorescence, Staining, Control