cell lines normal human epidermal melanocyte  (PromoCell)

Journal: Journal of Synchrotron Radiation

Article Title: Effects of synchrotron-based X-rays and gold nanoparticles on normal and cancer cell morphology and migration

doi: 10.1107/S1600577522012024

Figure Lengend Snippet: Human epidermal melanocytes HEM and human primary melanoma MM418-C1 monolayer 24 h after being exposed to 1000 Gy 90 kVp SBB (width 500 µm). Both cell types are treated with 1 m M AuNPs. The boundaries of the radiation fields are marked with orange lines. (A1) HEM immediately after exposure, (A2) 24 h, (A3) 48 h and (A4) 96 h after exposure. (B1) MM418-C1 immediately after exposure, (B1) 24 h, (B2) 48 h and (B4) 96 h after exposure.

Article Snippet: Moshi Geso), and human colorectal adenocarcinoma SW48 (ATCC ® CCL-231 TM ; Manassas, VA, USA) – along with two different normal cell lines – human epidermal melanocytes HEM (ATCC ® PCS-200–013 TM ; Manassas, VA, USA) and human primary colon epithelial CCD841 CoN (ATCC ® CRL-1790 TM ; Manassas, VA, USA).

Techniques:

Journal: International Journal of Molecular Sciences

Article Title: Potential Therapeutic Skin Microbiomes Suppressing Staphylococcus aureus -Derived Immune Responses and Upregulating Skin Barrier Function-Related Genes via the AhR Signaling Pathway

doi: 10.3390/ijms23179551

Figure Lengend Snippet: Functional microbiomes modulate ROS-induced keratinocyte apoptosis via dual AhR/Nrf2 signaling and induce epidermal terminal differentiation. ( a ) Heatmap showing AhR signaling, Nrf2 signaling, and skin barrier-related genes stratified by SA abundance. ( b ) Measurement of mRNA levels of AhR , ARNT , CYP1A1, and PTGS2 relative to 18S rRNA. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to control group (HaCaT). # p < 0.05, ### p < 0.001 between S. hominis R9 and C. avidum R-CH3. ( c ) Measurement of mRNA levels of NFE2L2 , NQO1, and HMOX1 relative to 18S rRNA. ** p < 0.01, *** p < 0.001 compared to control group (HaCaT). ### p < 0.001 between S. hominis R9 and C. avidum R-CH3. ( d ) Boxplots of gene expression values for FLG , LOR, and CLDN1 stratified by an abundance of C. avidum in the Pso cohort. ** p < 0.01 between S. hominis R9 and C. avidum R-CH3. ( e ) Measurement of mRNA levels of AHR , CYP1A1 , NFE2L2, and OVOL1 relative to 18S rRNA in SA and SA and C. avidum treated groups with or without AHR gene silencing in HaCaT cells. *** p < 0.001 compared to each si-control group. ( f ) Measurement of mRNA levels of FLG , LOR , IVL, and CLDN1 relative to 18S rRNA in SA and SA and C. avidum treated groups with or without AHR gene silencing in HaCaT cells. *** p < 0.001, ns: not-significant compared to each si-control group. ### p < 0.001 between SA treatment group and C. avidum R-CH3 treatment group after SA treatment in AHR -silenced HaCaT cells. Statistical significance was calculated using Bonferroni tests.

Article Snippet: The human skin keratinocyte cell line, HaCaT, was purchased from PromoCell (Heidelberg, Germany).

Techniques: Functional Assay, Control, Gene Expression